Functional domains of a zinc metalloprotease from Vibrio vulnificus
نویسندگان
چکیده
منابع مشابه
Procaspase-3 activation by a metalloprotease secreted from Vibrio vulnificus.
Vibrio vulnificus is a marine bacterium and a human pathogen capable of causing wound infection and septicemia. We previously showed that the metalloprotease vEP secreted by V. vulnificus activates prothrombin in vitro. To further investigate the ability of vEP to activate other zymogens, we used a mutant form of procaspase-3 which lacks the native cleavage sites as a zymogen. The mutant zymoge...
متن کاملIron-mediated regulation of metalloprotease VvpE production in Vibrio vulnificus.
In Vibrio vulnificus, the production of metalloprotease VvpE is controlled by Crp (cAMP-receptor protein) and SmcR (a quorum sensing regulator) at the transcription level, and by PilD-mediated type II general secretion system (TTGSS) at the extracellular secretion level. Iron is known to stimulate VvpE production but the related mechanisms remain unidentified. Iron stimulated vvpE transcription...
متن کاملChemical modification of Vibrio vulnificus metalloprotease with activated polyethylene glycol.
Vibrio vulnificus, an opportunistic human pathogen causing septicemia, produces a metalloprotease which is suspected to be a virulence determinant, but which is labile in vivo due to inactivation by alpha-macroglobulin. To obtain a derivative which is stable in vivo, the metalloprotease was modified with activated monomethoxy polyethylene glycol. The modified protease retained full activity to ...
متن کاملMetalloprotease is not essential for Vibrio vulnificus virulence in mice.
Previous work suggested that a metalloprotease, Vvp, may be a virulence factor of Vibrio vulnificus, which causes severe wound infection and septicemia in humans. To determine the role of Vvp in pathogenesis, we isolated an isogenic protease-deficient (PD) mutant of Vibrio vulnificus by in vivo allelic exchange. This PD mutant was as virulent as its parental strain in mice infected intraperiton...
متن کاملA recombinant metalloprotease antigen of Vibrio vulnificus elicits protective antibodies in a murine model.
AIMS To investigate whether Vibrio vulnificus metalloprotease (VvpE) can induce the production of specific anti-VvpE antibody to confer effective protection against Vibrio vulnificus infection and to evaluate the possibility of VvpE as a potential vaccine candidate against disease caused by V. vulnificus. METHODS AND RESULTS The gene encoding the 65-kDa VvpE of V. vulnificus was amplified by ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1997
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.179.23.7606-7609.1997